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LearnLS-C331259
antibody from LSBio
Targeting: CBX5
HP1, HP1-ALPHA, HP1Hs-alpha
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-C331259 - Provider product page
- Provider
- LSBio
- Product name
- CBX5 / HP1 Alpha Antibody LS-C331259
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CBX5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CBX5 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug CBX5 antibody. Western blot was performed from the immunoprecipitate using CBX5 antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug CBX5 antibody. Western blot was performed from the immunoprecipitate using CBX5 antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug CBX5 antibody. Western blot was performed from the immunoprecipitate using CBX5 antibodyat a dilition of 1:1000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug CBX5 antibody. Western blot was performed from the immunoprecipitate using CBX5 antibodyat a dilition of 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using CBX5 antibody at dilution of 1:200 (400x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer using CBX5 antibody at dilution of 1:200 (400x lens).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer using CBX5 antibody at dilution of 1:200 (400x lens).
