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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX60833 - Provider product page
- Provider
- GeneTex
- Product name
- pan-HP1 antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Store at -20¢XC; for long storage, store at -80¢XC. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of nuclear extracts from HeLa cells (20 μg) using pan-HP1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells using pan-HP1 antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10¡¦ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with pan-HP antibody at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with NIH3T3 extracts and 4 μg of pan-HP1 antibody. The precipitated DNA was detected by PCR with primers targeting to the rDNA promoter and a subtelomeric sequence of chromosome 19.
