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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- TA347091 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal HP1alpha/beta/gamma Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal HP1alpha/beta/gamma Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CBX5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 2.0 ug/ul
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, ? and ? diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1? and HP1? is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa cells were stained with the antibody against HP1a,?,? and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1a,?,? antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody against HP1a, ? and ?. QPCR was performed on the IPd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Image shows the relative enrichment as compared to a no antibody negative control ChIP.
- Validation comment
- Assay
