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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-59154 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal HP1 Alpha, Beta, and Gamma Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody directed against HP1alpha,beta and gamma. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure shows the relative enrichment as compared to a no antibody negative control ChIP.
