Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- STJ99039 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CBX5 antibody [3G2-H10-A6] (STJ99039)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Chromobox Protein Homolog 5 is suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of HP1 alpha in MCF7, 293T, HelaNE, 3T3, C6, COS7 and Mouse brain lysates and using HP1 alpha mouse mAb (1:1000 diluted).Predicted band size: 22KDa.Observed band size: 22KDa
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry stain of Hela using HP1 alpha mouse mAb (1:300).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma with HP1 alpha mouse mAb (3G2-H10-A6, 1:400 diluted ) , showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrat buffer (pH6.0).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA