Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HP1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is expected to cross-react with mouse, Arabidopsis suecica, Rye, and Triticum aestivum based on sequence homology. Suggested positive control: antigen standard for CBX5 (transient overexpression lysate), Hela whole cell extract.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 13 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of HP 1 alpha in HeLa whole cell lysate using Product # PA1-16942. 1 min. ECL exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HP1 alpha in HeLa whole cell lysate. Sample was incubated in HP1 alpha polyclonal antibody (Product # PA1-16942).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of HP1 alpha in 1 x 10^6 cells/mL HeLa cells. Samples were incubated in HP1 alpha polyclonal antibody (Product # PA1-16942) using a dilution of 1:500. Antibody (orange) and rIgG (AF488) isotype control (blue). Both antibodies were conjugated to Alexa Fluor 488.
