PA5-29879

antibody from Invitrogen Antibodies

Targeting: CBX5 HP1, HP1-ALPHA, HP1Hs-alpha

Antibody data

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Validation data

Product number: PA5-29879 - Provider product page
Provider: Invitrogen Antibodies
Product name: HP1 alpha Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HeLa, HeLa nucleus. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (80%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CBX5 protein structure - PA5-29879 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HP1 alpha Polyclonal Antibody (Product # PA5-29879). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HeLa nucleus. 12% SDS PAGE. HP1 alpha Polyclonal Antibody (Product # PA5-29879) diluted at 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Chromobox protein homolog 5 was achieved by transfecting HeLa with Chromobox protein homolog 5 specific siRNAs (Silencer® select Product # s23884, s23883). Western blot analysis (Fig. a) was performed using Nuclear enriched extracts from the Chromobox protein homolog 5 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with HP1 alpha Polyclonal Antibody (Product # PA5-29879, 1:10000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Chromobox protein homolog 5.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-HP1 alpha Polyclonal Antibody (Product # PA5-29879) and a 25 kDa band corresponding to Chromobox protein homolog 5 was observed across tested cell lines. Nuclear enriched extracts (40 µg lysate) of MOLT-4 (Lane 1), HeLa (Lane 2), SH-SY5Y (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), NIH/3T3 (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).