PA5-28342

antibody from Invitrogen Antibodies

Targeting: PDK1

Antibody data

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Validation data

PDK1 protein structure - PA5-28342 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using PDK1 Polyclonal Antibody (Product # PA5-28342). Sample (30 µg whole cell lysate). A: HeLa S3. B: MOLT4. 10% SDS PAGE. PDK1 Polyclonal Antibody (Product # PA5-28342) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PDK1 antibody [N1C3] detects PDK1 protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human PDK1 transfected 293T cells.10 % SDS-PAGE. PDK1 antibody [N1C3] PDK1 Polyclonal Antibody (Product # PA5-28342) dilution: 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Pyruvate dehydrogenase kinase isozyme 1 Polyclonal Antibody (Product # PA5-28342) and 49-51 kDa bands corresponding to Pyruvate dehydrogenase kinase isozyme 1 protein was observed. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), Mouse Heart (Lane 4), Mouse Liver (Lane 5), Rat Heart (Lane 6) and Rat Liver ( lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).