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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX22495 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22495, RRID:AB_367020
- Product name
- Pyruvate dehydrogenase kinase 1 / PDK1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot using GeneTex's affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 ?g of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4¢X C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection is recommended. Other detection systems will yield similar results.
