NB100-93302

antibody from Novus Biologicals

Targeting: EIF3B EIF3S9, PRT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-93302

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-93302, RRID:AB_1236904

Product name

Rabbit Polyclonal EIF3B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Unlike for cellular mRNAs and other viral internal ribosome entry sites (IRESs), the eIF3 subunit e is not required for the translational activity of the HCV IRES.

Panthu B, Denolly S, Faivre-Moskalenko C, Ohlmann T, Cosset FL, Jalinot P
The Journal of biological chemistry 2020 Feb 14;295(7):1843-1856

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Increased replicative fitness can lead to decreased drug sensitivity of hepatitis C virus.

Sheldon J, Beach NM, Moreno E, Gallego I, Piñeiro D, Martínez-Salas E, Gregori J, Quer J, Esteban JI, Rice CM, Domingo E, Perales C
Journal of virology 2014 Oct;88(20):12098-111

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EIF3B Antibody [NB100-93302] - Detection of Human EIF3S9/eIF3B on HeLa whole cell lysate using NB100-93302. EIF3S9/eIF3B was also immunoprecipitated by rabbit anti-EIF3S9/eIF3B antibody NB100-93301.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EIF3B Antibody [NB100-93302] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF3B antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: EIF3B Antibody [NB100-93302] - Detection of human eIF3B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3B antibody NB100-93302 used for IP at 3 ug per reaction. eIF3B was also immunoprecipitated by another rabbit anti-eIF3B antibody. For blotting immunoprecipitated eIF3B, NB100-93302 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF3B Antibody [NB100-93302] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiEIF3S9/eIF3B used at a dilution of 1:200 (1ug/ml). Detection: DAB