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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- PA5-23278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-eIF3b Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Human eIF3b and eIF3a serve as the nucleation core for the assembly of eIF3 into two interconnected modules: the yeast-like core and the octamer.
Wagner S, Herrmannová A, Šikrová D, Valášek LS
Nucleic acids research 2016 Dec 15;44(22):10772-10788
Nucleic acids research 2016 Dec 15;44(22):10772-10788
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using a eIF3B polyclonal antibody (Product # PA5-23278) at 0.25 µg/mL, 0.25 µg/mL and 0.05 µg/mL respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KD of eIF3b was achieved by transfecting A-431 with eIF3b specific siRNAs (Silencer® select Product # s16492, s16493). Western blot analysis (Fig. a) was performed using whole cell extracts from the eIF3b KD cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with eIF3b Polyclonal Antibody (Product # PA5-23278, 0.25 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to eIF3b. .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-eIF3b Polyclonal Antibody (Product # PA5-23278) and 110 kDa band corresponding to eIF3b was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), HEK-293 (Lane 3), NIH/3T3 (Lane 4), K-562 (Lane 5), Jurkat (Lane 6), DU 145 (Lane 7), PANC-1 (Lane 8) and SH-SY5Y (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.25 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
