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antibody from Invitrogen Antibodies
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- 702313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL12 Recombinant Rabbit Monoclonal Antibody (1H15L3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Pig, Cat and Sheep.
- Antibody clone number
- 1H15L3
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Indirect ELISA analysis of Anti-CXCL12/ SDF-1α Recombinant Rabbit Monoclonal Antibody (Product # 702313) was performed by coating recombinant human CXCL12 protein standard at concentrations ranging from 0.24 ng/mL to 250 ng/mL onto a plate. The coated protein was bound by Anti-CXCL12/ SDF-1α Recombinant Rabbit Monoclonal Antibody at 1 µg/mL and was detected using Goat anti-Rabbit IgG (H+L) secondary antibody, HRP-conjugate (Product # G-21234, 1:5000 dilution). The plate was developed using stabilized chromogen solution, TMB+H2O2 (Product # SB01). The plate was read at 450 nm with Thermo Scientific ™ Multiskan ™ GO microplate spectrophotometer. The endogenous levels of CXCL12/ SDF-1α in diseased human serum (Atopic dermatitis) and serum (pooled atopic dermatitis and Multiple sclerosis) along with simulated cell treatment (THP1+LPS @ 1 µg/mL for 24 hrs+PMA @ 100 nM for 10 mins) was quantitated by an indirect ELISA analysis by coating the samples onto the plate as per dilutions mentioned. The samples were detected as mentioned above. Note : The experiment was performed using an antibody pair buffer kit (Product # CNB0011).
