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LearnPA5-89116
antibody from Invitrogen Antibodies
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-89116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: ARANVKHLKI LNTPNCALQI VARLKNNNRQ VCIDPKLKWI QEYLEKALNK RFKM Positive Samples: NCI-H292
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.68 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
Eweida A, Flechtenmacher S, Sandberg E, Schulte M, Schmidt VJ, Kneser U, Harhaus L
PloS one 2022;17(8):e0272697
PloS one 2022;17(8):e0272697
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CXCL12 in THP-1 cells. Samples were incubated with CXCL12 Polyclonal antibody (Product # PA5-89116) using a dilution of 1:200. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded human stomach. Samples were incubated with CXCL12 Polyclonal antibody (Product # PA5-89116) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded human tonsil. Samples were incubated with CXCL12 Polyclonal antibody (Product # PA5-89116) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded rat spleen. Samples were incubated with CXCL12 Polyclonal antibody (Product # PA5-89116) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0272697.g005 Fig 5 Above: histomorphometric analysis of HIF-1 and SDF-1 expression within the constructs, (*) indicates statistical significance. Below: combined Immunofluorescence image of AVL construct showing the vascular axis (v), the homing cells labelled with QDots in green (arrows), the SDF-1 expression in red around the vessel (dotted arrows) and the cell nuclei in blue stained with Hoechst.
