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antibody from Invitrogen Antibodies
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-89116 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: ARANVKHLKI LNTPNCALQI VARLKNNNRQ VCIDPKLKWI QEYLEKALNK RFKM
- Concentration
- 2.89 mg/mL
Submitted references Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
Eweida A, Flechtenmacher S, Sandberg E, Schulte M, Schmidt VJ, Kneser U, Harhaus L
PloS one 2022;17(8):e0272697
PloS one 2022;17(8):e0272697
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of NCI-H292 cells, using CXCL12 Polyclonal antibody (Product # PA5-89116). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded human tonsil using CXCL12 Polyclonal Antibody (Product # PA5-89116) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded human stomach using CXCL12 Polyclonal Antibody (Product # PA5-89116) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded human tonsil using CXCL12 Polyclonal Antibody (Product # PA5-89116) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CXCL12 in paraffin-embedded rat spleen using CXCL12 Polyclonal Antibody (Product # PA5-89116) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of CXCL12 was performed in human liver cancer tissue using CXCL12 Polyclonal Antibody (Product # PA5-89116) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pone.0272697.g005 Fig 5 Above: histomorphometric analysis of HIF-1 and SDF-1 expression within the constructs, (*) indicates statistical significance. Below: combined Immunofluorescence image of AVL construct showing the vascular axis (v), the homing cells labelled with QDots in green (arrows), the SDF-1 expression in red around the vessel (dotted arrows) and the cell nuclei in blue stained with Hoechst.
