Explore
Validate
LearnPA5-30603
antibody from Invitrogen Antibodies
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCL12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A549. Predicted reactivity: Mouse (93%), Rat (92%), Dog (93%), Cat (95%), Pig (90%), Chicken (83%), Rhesus Monkey (97%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.16 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Chronic Prostatitis Affects Male Reproductive Health and Is Associated with Systemic and Local Epigenetic Inactivation of C-X-C Motif Chemokine 12 Receptor C-X-C Chemokine Receptor Type 4.
Schagdarsurengin U, Teuchert LM, Hagenkötter C, Nesheim N, Dansranjavin T, Schuppe HC, Gies S, Pilatz A, Weidner W, Wagenlehner FM
Urologia internationalis 2017;98(1):89-101
Urologia internationalis 2017;98(1):89-101
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SDF1 using anti-SDF1 Polyclonal Antibody (Product # PA5-30603), in A549 Cell Lysate (30 µg of cell lysate) and at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCL12 was performed by separating 30 µg of whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL12 Polyclonal Antibody (Product # PA5-30603) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CXCL12 Polyclonal Antibody (Product # PA5-30603). Various extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL12 Polyclonal Antibody (Product # PA5-30603) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCL12 Polyclonal Antibody detects SDF1 protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDF1 stained by CXCL12 Polyclonal Antibody (Product # PA5-30603) diluted at 1:50. Red: Tau, a Axon marker, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
