IC350F
antibody from R&D Systems
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC350F - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
Q6ICW0
- Isotype
- IgG
- Antibody clone number
- 79018
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Long-term B cell depletion in murine lupus eliminates autoantibody-secreting cells and is associated with alterations in the kidney plasma cell niche.
Wang W, Rangel-Moreno J, Owen T, Barnard J, Nevarez S, Ichikawa HT, Anolik JH
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3011-20
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3011-20
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCL12/SDF-1 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.