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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-61040 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61040, RRID:AB_925339
- Product name
- Rabbit Polyclonal CORO1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines.
Kimura T, Kaneko Y, Yamada S, Ishihara H, Senda T, Iwamatsu A, Niki I
Journal of cell science 2008 Sep 15;121(Pt 18):3092-8
Journal of cell science 2008 Sep 15;121(Pt 18):3092-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CORO1B Antibody [NB100-61040] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-61040 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Coronin 2 was also immunoprecipitated by rabbit anti-Coronin 2 antibody NB100-61039, which recognizes an upstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-Coronin 2 used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CORO1B Antibody [NB100-61040] - Human colon carcinoma. Antibody used at a dilution of 1:1,000.
