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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA1-20178 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Angiopoietin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Bone marrow pericyte dysfunction in individuals with type 2 diabetes.
Mangialardi G, Ferland-McCollough D, Maselli D, Santopaolo M, Cordaro A, Spinetti G, Sambataro M, Sullivan N, Blom A, Madeddu P
Diabetologia 2019 Jul;62(7):1275-1290
Diabetologia 2019 Jul;62(7):1275-1290
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 9 Inhibition of ANGPT2 by a blocking antibody restores the ability of pericytes from diabetic individuals to promote endothelial network formation. Pericytes from non-diabetic or diabetic individuals were treated with anti-ANGPT2 antibody or with IgG isotype control. Their conditioned media was harvested and used to stimulate the formation of networks by HUVECs on Matrigel. ( a , b ) Representative images ( a ) and bar graph ( b ) showing that conditioned media of pericytes from diabetic individuals inhibits endothelial network formation compared with conditioned media of pericytes from non-diabetic individuals. ( c , d ) Representative images ( c ) and bar graph ( d ) showing that the inhibition exerted by the conditioned media of pericytes from diabetic individuals is abrogated by an ANGPT2 blocking antibody. Bar and whisker graphs show mean, 25th-75th percentile and minimum and maximum values. n =3 per group. ** p
