Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB7186 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Angiopoietin-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Angiopoietin-1, rmAngiopoietin-3, recombinant human (rh) Angiopoietin-2, and rhAngiopoietin-4 is observed. In Western blots, 100% cross-reactivity with rmAngiopoietin-1 is observed and less than 10% cross-reactivity with rhAngiopoietin-2, rhAngiopoietin-4, and rmAngiopoietin-3 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis.
Retzlaff J, Thamm K, Ghosh CC, Ziegler W, Haller H, Parikh SM, David S
Scientific reports 2017 Mar 9;7:44113
Scientific reports 2017 Mar 9;7:44113
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Angiopoietin-2 by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse uterus tissue, and mouse ovary tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin-2 Monoclonal Antibody (Catalog # MAB7186) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
