Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB0983-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Angiopoietin-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, or rmANGPTL3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Angiopoietin-2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, human placenta tissue, human ovary tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Angiopoietin-2 Monoclonal Antibody (Catalog # MAB0983) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.