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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- MAB0983-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Angiopoietin-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, or rmANGPTL3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O15123- Isotype
- IgG
- Antibody clone number
- 180102
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Endothelial pyruvate kinase M2 maintains vascular integrity.
Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D.
Regulation of angiopoietin expression by bacterial lipopolysaccharide.
Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
Kim B, Jang C, Dharaneeswaran H, Li J, Bhide M, Yang S, Li K, Arany Z
The Journal of clinical investigation 2018 Oct 1;128(10):4543-4556
The Journal of clinical investigation 2018 Oct 1;128(10):4543-4556
Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D.
Demolli S, Doddaballapur A, Devraj K, Stark K, Manavski Y, Eckart A, Zehendner CM, Lucas T, Korff T, Hecker M, Massberg S, Liebner S, Kaluza D, Boon RA, Dimmeler S
Cardiovascular research 2017 May 1;113(6):681-691
Cardiovascular research 2017 May 1;113(6):681-691
Regulation of angiopoietin expression by bacterial lipopolysaccharide.
Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L955-63
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L955-63
Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta.
Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM
American journal of obstetrics and gynecology 2006 Feb;194(2):564-71
American journal of obstetrics and gynecology 2006 Feb;194(2):564-71
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Angiopoietin-2 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, human placenta tissue, human ovary tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Angiopoietin-2 Monoclonal Antibody (Catalog # MAB0983) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin-2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
