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- References [9]
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- Product number
- A00370-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Angiopoietin-2/ANGPT2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Angiopoietin-2/ANGPT2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references An oxidation responsive nano-radiosensitizer increases radiotherapy efficacy by remolding tumor vasculature.
Construction of an AuHQ nano-sensitizer for enhanced radiotherapy efficacy through remolding tumor vasculature.
Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation.
Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients.
Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes.
Expression of Ang-2/Tie-2 and PI3K/AKT in colorectal cancer.
The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery.
Exercise improves recovery after ischemic brain injury by inducing the expression of angiopoietin-1 and Tie-2 in rats.
Expression of heparanase and angiopoietin-2 in patients with endometriosis.
Wang X, Niu X, Sha W, Feng X, Yu L, Zhang Z, Wang W, Yuan Z
Biomaterials science 2021 Sep 14;9(18):6308-6324
Biomaterials science 2021 Sep 14;9(18):6308-6324
Construction of an AuHQ nano-sensitizer for enhanced radiotherapy efficacy through remolding tumor vasculature.
Wang X, Niu X, Zhang X, Zhang Z, Gao X, Wang W, Yuan Z
Journal of materials chemistry. B 2021 Jun 3;9(21):4365-4379
Journal of materials chemistry. B 2021 Jun 3;9(21):4365-4379
Expression of angiopoietins in central nervous system hemangioblastomas is associated with cyst formation.
Li X, Liu W, Liu J, Wang W, Zhang S, Chen J, Hui X
Neuroscience letters 2017 Feb 3;639:120-125
Neuroscience letters 2017 Feb 3;639:120-125
Local renin-angiotensin system is associated with bone mineral density of glucocorticoid-induced osteoporosis patients.
Shuai B, Yang YP, Shen L, Zhu R, Xu XJ, Ma C, Lv L, Zhao J, Rong JH
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 2015 Mar;26(3):1063-71
Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 2015 Mar;26(3):1063-71
Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes.
Bai X, Li X, Tian J, Zhou Z
PloS one 2014;9(4):e96117
PloS one 2014;9(4):e96117
Expression of Ang-2/Tie-2 and PI3K/AKT in colorectal cancer.
Zhang JH, Wang LH, Li XJ, Wang AP, Reng LQ, Xia FG, Yang ZP, Jiang J, Wang XD, Wen CY
Asian Pacific journal of cancer prevention : APJCP 2014;15(20):8651-6
Asian Pacific journal of cancer prevention : APJCP 2014;15(20):8651-6
The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery.
Hao L, Wang XG, Cheng JD, You SZ, Ma SH, Zhong X, Quan L, Luo B
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 Jul-Aug;23(4):217-23
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 Jul-Aug;23(4):217-23
Exercise improves recovery after ischemic brain injury by inducing the expression of angiopoietin-1 and Tie-2 in rats.
Zheng Q, Zhu D, Bai Y, Wu Y, Jia J, Hu Y
The Tohoku journal of experimental medicine 2011 Jul;224(3):221-8
The Tohoku journal of experimental medicine 2011 Jul;224(3):221-8
Expression of heparanase and angiopoietin-2 in patients with endometriosis.
Jingting C, Yangde Z, Yi Z, Mengxiong L, Rong Y, Yu Z, Guoqing P, Lixiu P
European journal of obstetrics, gynecology, and reproductive biology 2008 Feb;136(2):199-209
European journal of obstetrics, gynecology, and reproductive biology 2008 Feb;136(2):199-209
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 66KD. The expected band size for ANGPT2 is at 57KD.
- Additional image
