Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF623 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Angiopoietin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse Angiopoietin-2 is observed and less than 5% cross-reactivity with recombinant human Angiopoietin-1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O15123
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT.
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis.
Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma.
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Endothelial destabilization by angiopoietin-2 via integrin β1 activation.
Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma.
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways.
Norrin promotes vascular regrowth after oxygen-induced retinal vessel loss and suppresses retinopathy in mice.
Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
Lin CY, Cho CF, Bai ST, Liu JP, Kuo TT, Wang LJ, Lin YS, Lin CC, Lai LC, Lu TP, Hsieh CY, Chu CN, Cheng DC, Sher YP
Scientific reports 2017 Nov 8;7(1):15108
Scientific reports 2017 Nov 8;7(1):15108
Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis.
Retzlaff J, Thamm K, Ghosh CC, Ziegler W, Haller H, Parikh SM, David S
Scientific reports 2017 Mar 9;7:44113
Scientific reports 2017 Mar 9;7:44113
Association of Angiopoietin-2 and Ki-67 Expression with Vascular Density and Sunitinib Response in Metastatic Renal Cell Carcinoma.
Rautiola J, Lampinen A, Mirtti T, Ristimäki A, Joensuu H, Bono P, Saharinen P
PloS one 2016;11(4):e0153745
PloS one 2016;11(4):e0153745
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger MS, Wu D, Min W
Nature medicine 2016 Sep;22(9):1033-1042
Nature medicine 2016 Sep;22(9):1033-1042
Endothelial destabilization by angiopoietin-2 via integrin β1 activation.
Hakanpaa L, Sipila T, Leppanen VM, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nature communications 2015 Jan 30;6:5962
Nature communications 2015 Jan 30;6:5962
Angiopoietin-2 and biliary diseases: elevated serum, but not bile levels are associated with cholangiocarcinoma.
Voigtländer T, David S, Thamm K, Schlué J, Metzger J, Manns MP, Lankisch TO
PloS one 2014;9(5):e97046
PloS one 2014;9(5):e97046
Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes.
Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perrière N, Guillevic O, Couraud PO, Uzan G
PloS one 2014;9(1):e84179
PloS one 2014;9(1):e84179
Angiopoietin-2 secretion by endothelial cell exosomes: regulation by the phosphatidylinositol 3-kinase (PI3K)/Akt/endothelial nitric oxide synthase (eNOS) and syndecan-4/syntenin pathways.
Ju R, Zhuang ZW, Zhang J, Lanahan AA, Kyriakides T, Sessa WC, Simons M
The Journal of biological chemistry 2014 Jan 3;289(1):510-9
The Journal of biological chemistry 2014 Jan 3;289(1):510-9
Norrin promotes vascular regrowth after oxygen-induced retinal vessel loss and suppresses retinopathy in mice.
Ohlmann A, Seitz R, Braunger B, Seitz D, Bösl MR, Tamm ER
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Jan 6;30(1):183-93
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Jan 6;30(1):183-93
Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma.
Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D, Augustin HG
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1384-92
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Feb 15;15(4):1384-92
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Angiopoietin-2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Angiopoietin-2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-2 in Human Gastrointestinal Cancer Tissue. Angiopoietin-2 was detected in immersion fixed paraffin-embedded sections of human gastrointestinal cancer tissue using 15 µg/mL Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-2 in Human Liver Cancer Tissue. Angiopoietin-2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human Angiopoietin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF623) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.