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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00005467-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005467-M01, RRID:AB_425605
- Product name
- PPARD monoclonal antibody (M01), clone 4E3-1B11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PPARD.
- Antigen sequence
MEQPQEEAPEVREEEEKEEVAEAEGAPELNGGPQH
ALPSSSYTDLSRSSSPPSLLDQLQMGCDGASCGSL
NMECRVCGDKASGFHYGVHACEGCKGFFRRTIRMK
LEYEKCERSCKIQKKNRNKCQYCRFQKCLALGMSH
NAIRFGRMPEAEKRKLVAGLTANEGSQYNPQVADL
KAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAP
FVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHV
FYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVT
LLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTRE
FLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLAL
FIAAIILCGGE- Isotype
- IgG
- Antibody clone number
- 4E3-1B11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein.
Holloway GP, Perry CG, Thrush AB, Heigenhauser GJ, Dyck DJ, Bonen A, Spriet LL
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1060-9
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1060-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PPARD monoclonal antibody (M01), clone 4E3-1B11 Western Blot analysis of PPARD expression in Jurkat ( Cat # L017V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PPARD is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between HDAC2 and PPARD. HeLa cells were stained with anti-HDAC2 rabbit purified polyclonal 1:1200 and anti-PPARD mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
