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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- PAB11322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11322, RRID:AB_1710907
- Product name
- Ppard polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Ppard.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Transcriptional repression of atherogenic inflammation: modulation by PPARdelta.
Medicine. PPARs as therapeutic targets: reverse cardiology?
Progress in cardiovascular biology: PPAR for the course.
Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation.
Lee CH, Chawla A, Urbiztondo N, Liao D, Boisvert WA, Evans RM, Curtiss LK
Science (New York, N.Y.) 2003 Oct 17;302(5644):453-7
Science (New York, N.Y.) 2003 Oct 17;302(5644):453-7
Medicine. PPARs as therapeutic targets: reverse cardiology?
Plutzky J
Science (New York, N.Y.) 2003 Oct 17;302(5644):406-7
Science (New York, N.Y.) 2003 Oct 17;302(5644):406-7
Progress in cardiovascular biology: PPAR for the course.
Lazar MA
Nature medicine 2001 Jan;7(1):23-4
Nature medicine 2001 Jan;7(1):23-4
Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation.
Chinetti G, Fruchart JC, Staels B
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2000 Oct;49(10):497-505
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2000 Oct;49(10):497-505
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of Ppard polyclonal antibody (Cat # PAB11322) is shown to detect a predominant band at ~ 43 kDa corresponding to Ppard present in mouse heart tissue lysates.Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown).Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.Detection occurred using a 1 : 750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green).IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
