GTX28937

antibody from GeneTex

Targeting: PPARD FAAR, NR1C2, NUC1, NUCII

ELISA

Antibody data

Submit

Validation data

PPARD protein structure - GTX28937 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blotting. Affinity Purified Anti-PPAR delta (Rabbit) is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4° C followed reaction with a 1:10,000 dilution of IRDye® 800 conjugated goat anti-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western blotting. Affinity Purified Anti-PPAR delta (Rabbit) is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ?g of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4¢X C followed reaction with a 1:10,000 dilution of IRDye? 800 conjugated goat anti-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye? 800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.