R1577P

antibody from Acris Antibodies GmbH

Targeting: PPARD FAAR, NR1C2, NUC1, NUCII

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

R1577P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1577P, RRID:AB_1006256

Product name

anti PPAR-delta (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids near the amino terminus of Mouse PPAR delta.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

0.1 mg

Concentration

1.57 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blotting: Affinity Purified Anti- PPAR delta [Rabbit] is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1/750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4°C followed reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.