Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-637 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-637, RRID:AB_10003140
- Product name
- Rabbit Polyclonal PPAR delta/NR1C2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. PPAR delta
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice.
PPARβ/δ and γ in a rat model of Parkinson's disease: possible involvement in PD symptoms.
Yang Q, Sun S, Liu W, Liu Q, Wang J
Biochemical and biophysical research communications 2020 May 7;525(3):639-645
Biochemical and biophysical research communications 2020 May 7;525(3):639-645
PPARβ/δ and γ in a rat model of Parkinson's disease: possible involvement in PD symptoms.
Falcone R, Florio TM, Di Giacomo E, Benedetti E, Cristiano L, Antonosante A, Fidoamore A, Massimi M, Alecci M, Ippoliti R, Giordano A, Cimini A
Journal of cellular biochemistry 2015 May;116(5):844-55
Journal of cellular biochemistry 2015 May;116(5):844-55
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPAR delta/NR1C2 Antibody [NB600-637] - Detection a predominant band at 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4 C followed reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PPAR delta/NR1C2 Antibody [NB600-637] - Rabbit Anti-PPAR delta (N terminal specific).