PA5-94984

antibody from Invitrogen Antibodies

Targeting: PTN HBGF8, HBNF, NEGF1

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-94984

Provider

Invitrogen Antibodies

Product name

Pleiotrophin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: Rat Brain Tissue, Rat Kidney Tissue, MCF-7 Cell, HT1080 Cell, SMMC Cell. IHC: Human Mammary Cancer Tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Pleiotrophin (PTN) was performed on starved U-87MG WT concentrated culture media. Antibody-bead conjugate was prepared by adding 2 µg of Pleiotrophin Polyclonal Antibody (Product # PA5-94984) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~1 hour at 4 degree celcius. One mg of protein was incubated with the antibody-bead conjugate for ~1 hour at 4 degree celcius. Following centrifugation and multiple washes, 2% starting material (SM), 2% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown (below immunoblot). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Pleiotrophin in paraffin-embedded human mammary cancer tissue. Samples were incubated with Pleiotrophin polyclonal antibody (Product # PA5-94984).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Pleiotrophin (PTN) was performed on starved U-87MG WT concentrated culture media. Antibody-bead conjugate was prepared by adding 2 µg of Pleiotrophin Polyclonal Antibody (Product # PA5-94984) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~1 hour at 4 degree celcius. One mg of protein was incubated with the antibody-bead conjugate for ~1 hour at 4 degree celcius. Following centrifugation and multiple washes, 2% starting material (SM), 2% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown (below immunoblot). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.