AF3320

antibody from Novus Biologicals

Targeting: S100G CABP1, CABP9K, CALB3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF3320

Provider

Novus Biologicals

Product name

Goat Polyclonal Calbindin D/S100G Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Calbindin D in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice.

Zhang M, Wang Y, Geng J, Zhou S, Xiao B
Cell reports 2019 Feb 5;26(6):1419-1431.e4

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Cerebrospinal Fluid Calbindin D Concentration as a Biomarker of Cerebellar Disease Progression in Niemann-Pick Type C1 Disease.

Bradbury A, Bagel J, Sampson M, Farhat N, Ding W, Swain G, Prociuk M, O'Donnell P, Drobatz K, Gurda B, Wassif C, Remaley A, Porter F, Vite C
The Journal of pharmacology and experimental therapeutics 2016 Aug;358(2):254-61

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Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.

Usoskin D, Furlan A, Islam S, Abdo H, Lönnerberg P, Lou D, Hjerling-Leffler J, Haeggström J, Kharchenko O, Kharchenko PV, Linnarsson S, Ernfors P
Nature neuroscience 2015 Jan;18(1):145-53

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Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.

Di Giovannantonio LG, Di Salvio M, Omodei D, Prakash N, Wurst W, Pierani A, Acampora D, Simeone A
Development (Cambridge, England) 2014 Jan;141(2):377-88

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Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra.

Bifsha P, Yang J, Fisher RA, Drouin J
PLoS genetics 2014 Dec;10(12):e1004863

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Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.

Di Salvio M, Di Giovannantonio LG, Acampora D, Prosperi R, Omodei D, Prakash N, Wurst W, Simeone A
Nature neuroscience 2010 Dec;13(12):1481-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Calbindin D by Western Blot. Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Calbindin D by Simple WesternTM. Detection of Human Calbindin D by Simple WesternTM.