Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF3320 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Calbindin D Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Calbindin D in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P05937- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice.
Cerebrospinal Fluid Calbindin D Concentration as a Biomarker of Cerebellar Disease Progression in Niemann-Pick Type C1 Disease.
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra.
Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
Zhang M, Wang Y, Geng J, Zhou S, Xiao B
Cell reports 2019 Feb 5;26(6):1419-1431.e4
Cell reports 2019 Feb 5;26(6):1419-1431.e4
Cerebrospinal Fluid Calbindin D Concentration as a Biomarker of Cerebellar Disease Progression in Niemann-Pick Type C1 Disease.
Bradbury A, Bagel J, Sampson M, Farhat N, Ding W, Swain G, Prociuk M, O'Donnell P, Drobatz K, Gurda B, Wassif C, Remaley A, Porter F, Vite C
The Journal of pharmacology and experimental therapeutics 2016 Aug;358(2):254-61
The Journal of pharmacology and experimental therapeutics 2016 Aug;358(2):254-61
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Usoskin D, Furlan A, Islam S, Abdo H, Lönnerberg P, Lou D, Hjerling-Leffler J, Haeggström J, Kharchenko O, Kharchenko PV, Linnarsson S, Ernfors P
Nature neuroscience 2015 Jan;18(1):145-53
Nature neuroscience 2015 Jan;18(1):145-53
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
Di Giovannantonio LG, Di Salvio M, Omodei D, Prakash N, Wurst W, Pierani A, Acampora D, Simeone A
Development (Cambridge, England) 2014 Jan;141(2):377-88
Development (Cambridge, England) 2014 Jan;141(2):377-88
Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra.
Bifsha P, Yang J, Fisher RA, Drouin J
PLoS genetics 2014 Dec;10(12):e1004863
PLoS genetics 2014 Dec;10(12):e1004863
Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
Di Salvio M, Di Giovannantonio LG, Acampora D, Prosperi R, Omodei D, Prakash N, Wurst W, Simeone A
Nature neuroscience 2010 Dec;13(12):1481-8
Nature neuroscience 2010 Dec;13(12):1481-8
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Calbindin D by Western Blot. Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
