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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 656005 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656005, RRID:AB_2563528
- Product name
- Alexa Fluor® 488 anti-IRF7
- Antibody type
- Monoclonal
- Antigen
- Partial IRF7 recombinant protein (127-503 a.a.)
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antibody clone number
- 12G9A36
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Overnight starved Jurkat cells were treated with or without IFN-α for 24 hours, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). Cells were then stained with IRF7 (clone 12G9A36) Alexa Fluor® 488 (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (dashed line for unstimulated, dotted line for stimulated).
- Conjugate
- Green dye
