656008

antibody from BioLegend

Targeting: IRF7

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

656008

Provider

BioLegend

Proper citation

BioLegend Cat#656008, RRID:AB_2563531

Product name

Alexa Fluor® 647 anti-IRF7

Antibody type

Monoclonal

Antigen

Partial IRF7 recombinant protein (127-503 a.a.)

Reactivity

Human

Host

Mouse

Conjugate

Red dye

Antibody clone number

12G9A36

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Overnight starved Jurkat cells were treated with or without IFN-α for 24 hours, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). Cells were then stained with IRF7 (clone 12G9A36) Alexa Fluor® 647 (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated).

Conjugate

Red dye