Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-5375-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRF7 Monoclonal Antibody (RDP4ND4), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody RDP4ND4 recognizes human interferon regulatory factor 7 (IRF7), a 54 kDa member of the interferon regulatory transcription factor family. It is essential for the antiviral innate immune response and the activation of type I IFN genes. IRF7 is constitutively expressed in the cytoplasm of plasmacytoid dendritic cells, B cells and monocytes, but it is strongly induced in response to virus infection and interferon stimulation.
- Conjugate
- Yellow dye
- Antibody clone number
- RDP4ND4
- Concentration
- 5 µL/Test
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- LEFT, MIDDLE: Normal human peripheral blood cells were unstimulated (left) or stimulated overnight with Human IFN alpha 2a Recombinant Protein (Product # 111011) (middle). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol, with CD303a Monoclonal Antibody, PE-Cyanine7 (Product # 25-9818) and IRF7 Monoclonal Antibody, PE. Viable cells in the pDC gate, as determined by Fixable Viability Dye eFluor 506 (Product # 65-0866), were used for analysis. RIGHT: CD303a+ pDC cells stained with Rat IgG2a kappa Isotype Control, PE (Product # 12-4321) (blue histogram) or IRF7 Monoclonal Antibody, PE (Product # 12-5375) on unstimulated (orange histogram) or Human IFN alpha 2a Recombinant Protein (Product # 111011)-stimulated human peripheral blood cells (purple histogram).
- Conjugate
- Yellow dye