51-5375-42

antibody from Invitrogen Antibodies

Targeting: IRF7

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

51-5375-42

Provider

Invitrogen Antibodies

Product name

IRF7 Monoclonal Antibody (RDP4ND4), Alexa Fluor™ 647, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody RDP4ND4 recognizes human interferon regulatory factor 7 (IRF7), a 54 kDa member of the interferon regulatory transcription factor family. It is essential for the antiviral innate immune response and the activation of type I IFN genes. IRF7 is constitutively expressed in the cytoplasm of plasmacytoid dendritic cells, B cells and monocytes, but it is strongly induced in response to virus infection and interferon stimulation.

Conjugate

Red dye

Antibody clone number

RDP4ND4

Concentration

5 µL/Test

References

Submitted references

SIDT1 plays a key role in type I IFN responses to nucleic acids in plasmacytoid dendritic cells and mediates the pathogenesis of an imiquimod-induced psoriasis model.

Morell M, Varela N, Castillejo-López C, Coppard C, Luque MJ, Wu YY, Martín-Morales N, Pérez-Cózar F, Gómez-Hernández G, Kumar R, O'Valle F, Alarcón-Riquelme ME, Marañón C
EBioMedicine 2022 Feb;76:103808

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LEFT, MIDDDLE: Normal human peripheral blood cells were unstimulated (left) or stimulated overnight with Human IFN alpha 2a Recombinant Protein (Product # 111011) (middle). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol, with CD303a Monoclonal Antibody, PE-Cyanine7 (Product # 25-9818) and IRF7 Monoclonal Antibody, Alexa Fluor 647. Viable cells in the pDC gate, as determined by Fixable Viability Dye eFluor 506 (Product # 65-0866), were used for analysis. RIGHT: CD303a+ pDC cells stained with Rat IgG2a kappa Isotype Control, Alexa Fluor 647 (Product # 51-4321) (blue histogram) or IRF7 Monoclonal Antibody, Alexa Fluor 647 (Product # 51-5375) on unstimulated (orange histogram) or Human IFN alpha 2a Recombinant Protein (Product # 111011)-stimulated human peripheral blood cells (purple histogram).

Conjugate

Red dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol, with CD303a Monoclonal Antibody, PE-Cyanine7 (Product # 25-9818) and Rat IgG2a kappa Isotype Control, Alexa Fluor 647 (Product # 51-4321) (left) or IRF7 Monoclonal Antibody, Alexa Fluor 647 (right). Cells in the pDC gate were used for analysis.

Conjugate

Red dye