PA5-38087

antibody from Invitrogen Antibodies

Targeting: LIPE HSL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-38087

Provider

Invitrogen Antibodies

Product name

Anti-Phospho-HSL (Ser554, Ser855) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-HSL pSer554 + pSer855 in extracts from HeLa cells treated with Adriamycin (0.5 ng/mL, 24hrs) using a Phospho-HSL pSer554 + pSer855 polyclonal antibody (Product # PA5-38087).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), 3T3-L1 differentiated to adipocytes treated with Doxorubicin (0.5ng/ml for 24h) (Lane 3) and Rat Adipose (Lane 4). The blot was probed with Anti-Phospho-HSL (Ser554, Ser855) Polyclonal Antibody (Product # PA5-38087, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). An 80kDa band corresponding to Phospho-HSL (Ser554, Ser855) was observed across all cell lines and tissues tested and was observed to be induced upon the differentiation of 3T3L1 cells to Adipocytes and upon treatment of the differentiated adipocytes with Doxorubicin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-HSL pSer554 + pSer855 in paraffin-embedded human colon carcinoma using a Phospho-HSL pSer554 + pSer855 polyclonal antibody (Product # PA5-38087).