Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-17488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HSL (Ser563) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Mutation of the novel acetylation site at K414R of BECN1 is involved in adipocyte differentiation and lipolysis.
Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.
Secretion of autoimmune antibodies in the human subcutaneous adipose tissue.
Extramitochondrial OPA1 and adrenocortical function.
Li C, Xu J, Yu Q, Wang P, Dong B, Shen L, Wang Q, Li S, Yang Y, Deng Y
Journal of cellular and molecular medicine 2021 Jul;25(14):6855-6863
Journal of cellular and molecular medicine 2021 Jul;25(14):6855-6863
Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.
An M, Park YH, Lim YH
Scientific reports 2021 Jan 28;11(1):2481
Scientific reports 2021 Jan 28;11(1):2481
Secretion of autoimmune antibodies in the human subcutaneous adipose tissue.
Frasca D, Diaz A, Romero M, Thaller S, Blomberg BB
PloS one 2018;13(5):e0197472
PloS one 2018;13(5):e0197472
Extramitochondrial OPA1 and adrenocortical function.
Fülöp L, Rajki A, Katona D, Szanda G, Spät A
Molecular and cellular endocrinology 2013 Dec 5;381(1-2):70-9
Molecular and cellular endocrinology 2013 Dec 5;381(1-2):70-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HSL pSer563 in extracts from differentiated 3T3-L1 cells treated with isoproterenol or lambda protein phosphatase using Phospho-HSL pSer563 polyclonal antibody (Product # PA5-17488).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HSL (Ser563) using a polyclonal antibody (Product # PA5-17488).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HSL pSer563 in 3T3-L1 adipocytes, isoproterenol-treated, using a Phospho-HSL pSer563 polyclonal antibody (Product # PA5-17488) (red). Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 5 Measure of lipolysis in the obese SAT versus blood. A. Adipocytes (AD), SVF and PBMC (blood) were sonicated for cell disruption in the presence of TRIzol to separate the soluble fraction (used for RNA isolation) from lipids and cell debris. AD and SVF were from the same obese individuals. PBMC (blood) were from obese individuals age-, gender- and BMI-matched. Results show qPCR values (2 -DeltaDeltaCt ) of LPL RNA expression. B. Total protein lysates of adipocytes (AD) and SVF (from different obese individuals age-, gender- and BMI-matched) were prepared and run in WB to measure phospho-HSL. A representative WB for the higest and lowest values is shown (top). Mean comparisons between groups were performed by Student's t test (two-tailed). ***p
