Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-17196 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 8 µg/mL
- Storage
- -20°C
Submitted references Adropin Slightly Modulates Lipolysis, Lipogenesis and Expression of Adipokines but Not Glucose Uptake in Rodent Adipocytes.
Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.
Jasaszwili M, Pruszyńska-Oszmałek E, Wojciechowicz T, Strowski MZ, Nowak KW, Skrzypski M
Genes 2021 Jun 13;12(6)
Genes 2021 Jun 13;12(6)
Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.
An M, Park YH, Lim YH
Scientific reports 2021 Jan 28;11(1):2481
Scientific reports 2021 Jan 28;11(1):2481
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSL in extracts from NIH/3T3 and 3T3-L1 cells using HSL polyclonal antibody (Product # PA5-17196).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), NIH/3T3 (Lane 3), NTERA-2 (Lane 4), Daudi (Lane 5), Mouse Kidney (Lane 6), Mouse Liver (Lane 7), Mouse Adipose (Lane 8) and Mouse Testis (Lane 9). The blot was probed with Anti-HSL Polyclonal Antibody (Product # PA5-17196, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). An 80kDa band corresponding to the short form of HSL was observed across all cell lines and tissues tested except for NIH/3T3, Daudi, Mouse Kidney and Mouse Liver. The 120kDa long form of HSL, which is only reported in steroidogenic tissues, was observed in Mouse Testis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HSL in 3T3-L1 adipocytes using a HSL polyclonal antibody (Product # PA5-17196) (red). Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Effects of MJ2 and hkMJ2 on protein expression levels related to adipogenesis and lipid metabolism in eWAT. Total protein was extracted from eWAT in each group, and the relative protein expression levels of the factors related to ( A ) adipogenesis (PPARgamma and C/EBPalpha), ( B ) lipogenesis (FAS, SCD-1 and ACC) and ( C ) lipolysis (ATGL and HSL) were investigated by western blot. Representative images of each protein are shown, and the relative quantified expression levels indicate the mean +- SD. The p values were determined by ANOVA and Tukey's HSD test. The full-length blots are shown in Supplementary Fig. 3 .