PA5-26383

antibody from Invitrogen Antibodies

Targeting: LIPE HSL

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-26383

Provider

Invitrogen Antibodies

Product name

HSL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Secretion of autoimmune antibodies in the human subcutaneous adipose tissue.

Frasca D, Diaz A, Romero M, Thaller S, Blomberg BB
PloS one 2018;13(5):e0197472

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HL-60 cells using a LIPE polyclonal antibody (Product # PA5-26383) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 Measure of lipolysis in the obese SAT versus blood. A. Adipocytes (AD), SVF and PBMC (blood) were sonicated for cell disruption in the presence of TRIzol to separate the soluble fraction (used for RNA isolation) from lipids and cell debris. AD and SVF were from the same obese individuals. PBMC (blood) were from obese individuals age-, gender- and BMI-matched. Results show qPCR values (2 -DeltaDeltaCt ) of LPL RNA expression. B. Total protein lysates of adipocytes (AD) and SVF (from different obese individuals age-, gender- and BMI-matched) were prepared and run in WB to measure phospho-HSL. A representative WB for the higest and lowest values is shown (top). Mean comparisons between groups were performed by Student's t test (two-tailed). ***p