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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [4]
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- Product number
- MA1-06800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD56 Monoclonal Antibody (RNL-1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-06800 detects NCAM in human and mouse samples.
- Antibody clone number
- RNL-1
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82 using CD56/NCAM monoclonal antibody (Product # MA1-06800)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82 using CD56/NCAM monoclonal antibody (Product # MA1-06800)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CD56 in H82 cells, derived from small cell lung cancer, using a CD56 Monoclonal Antibody (RNL-1) (Product # MA1-06800) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD56 Monoclonal Antibody (RNL-1) (Product # MA1-06800) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Product # A32787), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents HepG2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
