MA1-06801

antibody from Invitrogen Antibodies

Targeting: NCAM1 CD56, NCAM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-06801

Provider

Invitrogen Antibodies

Product name

CD56 Monoclonal Antibody (123C3)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-06801 detects NCAM in human samples.

Antibody clone number

123C3

Concentration

1 mg/mL

References

Submitted references

The anti-B7-H4 checkpoint synergizes trastuzumab treatment to promote phagocytosis and eradicate breast cancer.

Hu X, Liu Y, Zhang X, Kong D, Kong J, Zhao D, Guo Y, Sun L, Chu L, Liu S, Hou X, Ren F, Zhao Y, Lu C, Zhai D, Yuan X
Neoplasia (New York, N.Y.) 2020 Nov;22(11):539-553

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Clinicopathological and Preclinical Findings of NUT Carcinoma: A Multicenter Study.

Jung M, Kim S, Lee JK, Yoon SO, Park HS, Hong SW, Park WS, Kim JE, Kim J, Keam B, Kim HJ, Kang HJ, Kim DW, Jung KC, Kim YT, Heo DS, Kim TM, Jeon YK
The oncologist 2019 Aug;24(8):e740-e748

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Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Su S, Zhao J, Xing Y, Zhang X, Liu J, Ouyang Q, Chen J, Su F, Liu Q, Song E
Cell 2018 Oct 4;175(2):442-457.e23

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Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma.

Weil S, Memmer S, Lechner A, Huppert V, Giannattasio A, Becker T, Müller-Runte A, Lampe K, Beutner D, Quaas A, Schubert R, Herrmann E, Steinle A, Koehl U, Walter L, von Bergwelt-Baildon MS, Koch J
Frontiers in immunology 2017;8:387

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Usefulness of the octreotide test in Japanese patients for predicting the presence/absence of somatostatin receptor 2 expression in insulinomas.

Nakamura A, Mitsuhashi T, Takano Y, Miyoshi H, Kameda H, Nomoto H, Nagai S, Hatanaka Y, Shimizu C, Terauchi Y, Atsumi T
Endocrine journal 2016;63(2):135-42

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CD56, HBME-1 and cytokeratin 19 expressions in papillary thyroid carcinoma and nodular thyroid lesions.

Erdogan-Durmus S, Ozcan D, Yarikkaya E, Kurt A, Arslan A
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences 2016;21:49

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Role of cd56 and e-cadherin expression in the differential diagnosis of papillary thyroid carcinoma and suspected follicular-patterned lesions of the thyroid: the prognostic importance of e-cadherin.

Ceyran AB, Şenol S, Şimşek BÇ, Sağıroğlu J, Aydın A
International journal of clinical and experimental pathology 2015;8(4):3670-80

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Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis.

Marković-Lipkovski J, Životić M, Müller CA, Tampe B, Ćirović S, Vještica J, Tomanović N, Zeisberg M, Müller GA
PloS one 2015;10(9):e0137028

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Reevaluation and reclassification of resected lung carcinomas originally diagnosed as squamous cell carcinoma using immunohistochemical analysis.

Kadota K, Nitadori J, Rekhtman N, Jones DR, Adusumilli PS, Travis WD
The American journal of surgical pathology 2015 Sep;39(9):1170-80

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Canalicular adenoma: a clinicopathologic and immunohistochemical analysis of 67 cases with a review of the literature.

Thompson LD, Bauer JL, Chiosea S, McHugh JB, Seethala RR, Miettinen M, Müller S
Head and neck pathology 2015 Jun;9(2):181-95

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Pagetoid reticulosis tumor cells with double expression of TCRγδ and TCRαβ: an off-target phenomenon or genuine expression?

Tomasini D, Niccoli A, Crivelli F
Journal of cutaneous pathology 2015 Jun;42(6):427-34

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Immunological significance of the accumulation of autophagy components in oral squamous cell carcinoma.

Sakakura K, Takahashi H, Kaira K, Toyoda M, Oyama T, Chikamatsu K
Cancer science 2015 Jan;106(1):1-8

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CD30(+) extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Yang YJ, Li YX, Liu YB, Yang M, Liu K
Oncology letters 2014 May;7(5):1419-1421

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Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature.

Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher RF, Cabassa P, Facchetti F
International journal of surgical pathology 2014 Dec;22(8):712-21

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A Case of Distal Epithelioid Sarcoma of the Thumb Expressing Podoplanin, TLE1 and Ca 125.

Karagkounis G, Argyrakos T, Charkiolakis G, Castana O, Rontogianni D
Case reports in pathology 2013;2013:312786

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Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Su XY, Huang J, Jiang Y, Tang Y, Li GD, Liu WP
Cytopathology : official journal of the British Society for Clinical Cytology 2012 Apr;23(2):96-102

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Extraosseous (extramedullary) plasmacytomas: a clinicopathologic and immunophenotypic study of 32 Chinese cases.

Zuo Z, Tang Y, Bi CF, Zhang WY, Zhao S, Wang XQ, Yang QP, Zou LQ, Liu WP
Diagnostic pathology 2011 Dec 19;6:123

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Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Lee DW, Yang JH, Lee SM, Won CH, Chang S, Lee MW, Choi JH, Moon KC
Annals of dermatology 2011 Aug;23(3):329-37

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Primary central nervous system peripheral T-cell lymphoma in a child.

Gualco G, Wludarski S, Hayashi-Silva L, Medeiros Filho P, Veras G, Bacchi CE
Fetal and pediatric pathology 2010;29(4):224-30

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Primary plasmacytoma of the cervix in a 21-year-old female patient.

Schor AP, Moraes MP, Bisson FW, Bisson MA, Luiz OM, Bacchi CE
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2010 May;29(3):290-3

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Atypical carcinoid with prominent mucinous stroma: a hitherto unreported variant of pulmonary neuroendocrine tumor.

Nannini N, Bertolini F, Cavazza A, Casali C, Mengoli MC, Rossi G
Endocrine pathology 2010 Jun;21(2):120-4

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Goblet cell carcinoid of the appendix: a clinicopathological and immunohistochemical study of 26 cases from southwest china.

Yong Jiang, Huawei Liu, Hu Long, Yingying Yang, Dianying Liao, Xiuhui Zhang
International journal of surgical pathology 2010 Dec;18(6):488-92

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Composite lymphoma: EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma: a case report.

Gualco G, Chioato L, Van Den Berg A, Weiss LM, Bacchi CE
Applied immunohistochemistry & molecular morphology : AIMM 2009 Jan;17(1):72-6

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Composite lymphoma: EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma: a case report.

Gualco G, Chioato L, Van Den Berg A, Weiss LM, Bacchi CE
Applied immunohistochemistry & molecular morphology : AIMM 2009 Jan;17(1):72-6

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EGFR and K-ras mutations along the spectrum of pulmonary epithelial tumors of the lung and elaboration of a combined clinicopathologic and molecular scoring system to predict clinical responsiveness to EGFR inhibitors.

Sartori G, Cavazza A, Sgambato A, Marchioni A, Barbieri F, Longo L, Bavieri M, Murer B, Meschiari E, Tamberi S, Cadioli A, Luppi F, Migaldi M, Rossi G
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The prognostic role of c-kit protein expression in resected large cell neuroendocrine carcinoma of the lung.

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The Annals of thoracic surgery 2004 Jan;77(1):247-52; discussion 252-3

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The prognostic role of c-kit protein expression in resected large cell neuroendocrine carcinoma of the lung.

Casali C, Stefani A, Rossi G, Migaldi M, Bettelli S, Parise A, Morandi U
The Annals of thoracic surgery 2004 Jan;77(1):247-52; discussion 252-3

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TTF-1, cytokeratin 7, 34betaE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung.

Rossi G, Marchioni A, Milani M, Scotti R, Foroni M, Cesinaro A, Longo L, Migaldi M, Cavazza A
American journal of clinical pathology 2004 Dec;122(6):884-93

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Desmoplastic and spindle cell melanomas express protein markers of the neural crest but not of later committed stages of Schwann cell differentiation.

Huttenbach Y, Prieto VG, Reed JA
Journal of cutaneous pathology 2002 Oct;29(9):562-8

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Desmoplastic and spindle cell melanomas express protein markers of the neural crest but not of later committed stages of Schwann cell differentiation.

Huttenbach Y, Prieto VG, Reed JA
Journal of cutaneous pathology 2002 Oct;29(9):562-8

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T-cell-rich large-B-cell lymphomas contain non-activated CD8+ cytolytic T cells, show increased tumor cell apoptosis, and have lower Bcl-2 expression than diffuse large-B-cell lymphomas.

Felgar RE, Steward KR, Cousar JB, Macon WR
The American journal of pathology 1998 Dec;153(6):1707-15

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CD56 Monoclonal Antibody (123C3)(Product # MA1-06801) and a ~150kDa band corresponding to CD56 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), Hep G2 (Lane 5), Neuro-2a (Lane 6), PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunostaining of the neural cell adhesion molecule (NCAM) in small cell lung cancer cell line NCI-H82 using CD56/NCAM monoclonal antibody (Product # MA1-06801)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD56 Monoclonal Antibody (123C3) (Product # MA1-06801) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents HepG2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 9 days old zebrafish embryo using CD56/NCAM monoclonal antibody (Product # MA1-06801).