- MA5-16446 - Invitrogen Antibodies NCAM1 antibody

Submitted references Usefulness of the octreotide test in Japanese patients for predicting the presence/absence of somatostatin receptor 2 expression in insulinomas.

Nakamura A, Mitsuhashi T, Takano Y, Miyoshi H, Kameda H, Nomoto H, Nagai S, Hatanaka Y, Shimizu C, Terauchi Y, Atsumi T
Endocrine journal 2016;63(2):135-42

Role of cd56 and e-cadherin expression in the differential diagnosis of papillary thyroid carcinoma and suspected follicular-patterned lesions of the thyroid: the prognostic importance of e-cadherin.

Ceyran AB, Şenol S, Şimşek BÇ, Sağıroğlu J, Aydın A
International journal of clinical and experimental pathology 2015;8(4):3670-80

Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis.

Marković-Lipkovski J, Životić M, Müller CA, Tampe B, Ćirović S, Vještica J, Tomanović N, Zeisberg M, Müller GA
PloS one 2015;10(9):e0137028

CD30(+) extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Yang YJ, Li YX, Liu YB, Yang M, Liu K
Oncology letters 2014 May;7(5):1419-1421

A Case of Distal Epithelioid Sarcoma of the Thumb Expressing Podoplanin, TLE1 and Ca 125.

Karagkounis G, Argyrakos T, Charkiolakis G, Castana O, Rontogianni D
Case reports in pathology 2013;2013:312786

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Su XY, Huang J, Jiang Y, Tang Y, Li GD, Liu WP
Cytopathology : official journal of the British Society for Clinical Cytology 2012 Apr;23(2):96-102

Extraosseous (extramedullary) plasmacytomas: a clinicopathologic and immunophenotypic study of 32 Chinese cases.

Zuo Z, Tang Y, Bi CF, Zhang WY, Zhao S, Wang XQ, Yang QP, Zou LQ, Liu WP
Diagnostic pathology 2011 Dec 19;6:123

Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Lee DW, Yang JH, Lee SM, Won CH, Chang S, Lee MW, Choi JH, Moon KC
Annals of dermatology 2011 Aug;23(3):329-37

Primary plasmacytoma of the cervix in a 21-year-old female patient.

Schor AP, Moraes MP, Bisson FW, Bisson MA, Luiz OM, Bacchi CE
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2010 May;29(3):290-3

Goblet cell carcinoid of the appendix: a clinicopathological and immunohistochemical study of 26 cases from southwest china.

Yong Jiang, Huawei Liu, Hu Long, Yingying Yang, Dianying Liao, Xiuhui Zhang
International journal of surgical pathology 2010 Dec;18(6):488-92

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CD56 Monoclonal Antibody (123C3) (Product # MA5-16446) and a 150 kDa band corresponding to CD56 was observed in SH-SY5Y and SK-N-SH. Membrane enriched cell extracts (12.5 µg lysate) of SH-SY5Y-Untreated (Lane 1), SH-SY5Y-Control (50°C, 3 hrs) (Lane 2), SH-SY5Y-Treated (500U PNGaseF; 50°C, 3 hr) (Lane 3), SK-N-SH-Untreated (Lane 4), SK-N-SH-Control (50°C, 3 hrs) (Lane 5) and SK-N-SH -Treated (500U PNGaseF; 50°C, 3 hr) (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:20,000 dilution) using the iBright FL1500 (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). CD56 migrates at ~150 kDa due to multiple glycosylations (Lane 1 and Lane 4). To carry out deglycosylation of the protein, PNGase F Glycan Cleavage Kit was used (Product # A39245). Upon PNGaseF treatment, N-linked glycosylations are cleaved and deglycosylated CD56 can be seen at ~120 kDa (Lane 3 and Lane 6). This mass shift can be attributed to deglycosylation as the control sample

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CD56 Monoclonal Antibody (123C3) (Product # MA5-16445, MA5-16446) and bands in the range of 130-240kDa corresponding to Neural cell adhesion molecule 1 were observed.. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), SK-N-SH (Lane 4), MCF7 (Lane 5), SK-BR-3 (Lane 6) and T-47D (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20,000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Relative expression was observed between SH-SY5Y, IMR-32, NK-92, SK-N-SH and breast cancer cell lines such as MCF7, SK-BR-3 and T-47D as expected (DOI: 10.1038/s41598-019-45377-8).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Neural cell adhesion molecule 1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (123C3) (Product # MA5-16445, MA5-16446) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents the merged image of MCF7 cells having very low to nil expression of CD56 as expected (DOI: 10.1038/s41598-019-45377-8). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MA5-16446

Antibody data