Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-33132 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NCAM-1/CD56 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
- Reactivity
- Human, Rat, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nano-Pulse Stimulation Ablates Orthotopic Rat Hepatocellular Carcinoma and Induces Innate and Adaptive Memory Immune Mechanisms that Prevent Recurrence.
Lassiter BP, Guo S, Beebe SJ
Cancers 2018 Mar 13;10(3)
Cancers 2018 Mar 13;10(3)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Formalin-fixed paraffin-embedded human colon Ganglion stained with CD56 Monoclonal antibody (123C3.D5)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - Analysis of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 Antibody (123C3) NBP2-33132AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes.Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - A surface stain was performed on U-87 Cells with NCAM-1/CD56 Antibody (123C3) NBP2-33132B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) - Azide and BSA Free [NBP2-33132] - analysis of CD56 on human monocytes using CD56 antibody at 1 ug/10^6 cells.