Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB7820 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal NCAM-1/CD56 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse NCAM-1/CD56 in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The prostaglandin E2 receptor EP3 controls CC-chemokine ligand 2-mediated neuropathic pain induced by mechanical nerve damage.
Treutlein EM, Kern K, Weigert A, Tarighi N, Schuh CD, Nüsing RM, Schreiber Y, Ferreirós N, Brüne B, Geisslinger G, Pierre S, Scholich K
The Journal of biological chemistry 2018 Jun 22;293(25):9685-9695
The Journal of biological chemistry 2018 Jun 22;293(25):9685-9695
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse NCAM-1/CD56 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Mouse NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB7820) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NCAM-1/CD56 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of NCAM-1/CD56 in Neuro-2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 Monoclonal Antibody (Catalog # MAB7820, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.