Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-34349-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NCAM-1/CD56 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
- Reactivity
- Human, Rat, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-fixed, paraffin-embedded human Cerebellum stained with CD56 Monoclonal Antibody (SPM128)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-paraffin human colon ganglion stained with CD56 MAb (SPM128)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (SPM128)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 (SPM128) antibody NBP2-34397APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) - Azide and BSA Free [NBP2-34397] - A surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 Antibody (SPM128) NBP2-34397APC (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Azide and BSA Free form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - A surface stain was performed on U-87 MG cells with NCAM-1/CD56 Antibody (SPM128) NBP2-34397AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (SPM128) [NBP2-34349] - FACS analysis of CD56 on human Monocytes using NCAM-1/CD56 Antibody (SPM128)