Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 61-0567-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD56 (NCAM) Monoclonal Antibody (CMSSB), PE-eFluor 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This CMSSB monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells. Staining with CMSSB does not block binding of MEM188 or CB56. Applications Reported: This CMSSB antibody has been reported for use in flow cytometric analysis. Applications Tested: This CMSSB antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CMSSB
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells.
In-depth immunophenotyping of patients with glioblastoma multiforme: Impact of steroid treatment.
Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.
Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.
Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.
Klöss V, Grünvogel O, Wabnitz G, Eigenbrod T, Ehrhardt S, Lasitschka F, Lohmann V, Dalpke AH
Frontiers in immunology 2017;8:1238
Frontiers in immunology 2017;8:1238
In-depth immunophenotyping of patients with glioblastoma multiforme: Impact of steroid treatment.
Chitadze G, Flüh C, Quabius ES, Freitag-Wolf S, Peters C, Lettau M, Bhat J, Wesch D, Oberg HH, Luecke S, Janssen O, Synowitz M, Held-Feindt J, Kabelitz D
Oncoimmunology 2017;6(11):e1358839
Oncoimmunology 2017;6(11):e1358839
Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.
Soh KT, Tario JD Jr, Colligan S, Maguire O, Pan D, Minderman H, Wallace PK
Current protocols in cytometry 2016 Jan 6;75:7.45.1-7.45.33
Current protocols in cytometry 2016 Jan 6;75:7.45.1-7.45.33
Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.
Bhanu NV, Sidoli S, Garcia BA
Proteomics 2016 Feb;16(3):448-58
Proteomics 2016 Feb;16(3):448-58
Innate lymphoid cells promote anatomical containment of lymphoid-resident commensal bacteria.
Sonnenberg GF, Monticelli LA, Alenghat T, Fung TC, Hutnick NA, Kunisawa J, Shibata N, Grunberg S, Sinha R, Zahm AM, Tardif MR, Sathaliyawala T, Kubota M, Farber DL, Collman RG, Shaked A, Fouser LA, Weiner DB, Tessier PA, Friedman JR, Kiyono H, Bushman FD, Chang KM, Artis D
Science (New York, N.Y.) 2012 Jun 8;336(6086):1321-5
Science (New York, N.Y.) 2012 Jun 8;336(6086):1321-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0036-42) and Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or Anti-Human CD56 (NCAM) PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.