- 14-9118-82 - Invitrogen Antibodies NCAM1 antibody

Submitted references Neurogenesis of medium spiny neurons in the nucleus accumbens continues into adulthood and is enhanced by pathological pain.

García-González D, Dumitru I, Zuccotti A, Yen TY, Herranz-Pérez V, Tan LL, Neitz A, García-Verdugo JM, Kuner R, Alfonso J, Monyer H
Molecular psychiatry 2021 Sep;26(9):4616-4632

Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice.

Polis B, Srikanth KD, Gurevich V, Bloch N, Gil-Henn H, Samson AO
International journal of molecular sciences 2020 Feb 8;21(3)

Bisecting GlcNAc Is a General Suppressor of Terminal Modification of N-glycan.

Nakano M, Mishra SK, Tokoro Y, Sato K, Nakajima K, Yamaguchi Y, Taniguchi N, Kizuka Y
Molecular & cellular proteomics : MCP 2019 Oct;18(10):2044-2057

Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures from Adult Mice.

Xiao L, Saiki C, Okamura H
International journal of molecular sciences 2019 Apr 15;20(8)

Human Dental Pulp Cells Differentiate toward Neuronal Cells and Promote Neuroregeneration in Adult Organotypic Hippocampal Slices In Vitro.

Xiao L, Ide R, Saiki C, Kumazawa Y, Okamura H
International journal of molecular sciences 2017 Aug 11;18(8)

Two separate subtypes of early non-subplate projection neurons in the developing cerebral cortex of rodents.

Espinosa A, Gil-Sanz C, Yanagawa Y, Fairén A
Frontiers in neuroanatomy 2009;3:27

Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer.

Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P
Science (New York, N.Y.) 2001 Apr 27;292(5517):740-3

Removal of polysialic acid-neural cell adhesion molecule induces aberrant mossy fiber innervation and ectopic synaptogenesis in the hippocampus.

Seki T, Rutishauser U
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 May 15;18(10):3757-66

Highly polysialylated neural cell adhesion molecule (NCAM-H) is expressed by newly generated granule cells in the dentate gyrus of the adult rat.

Seki T, Arai Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 1993 Jun;13(6):2351-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-PSA-NCAM Monoclonal Antibody (12E3), eBioscience(Product # 14-9118-82) and a ~150kDa band corresponding to PSA-NCAM was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), Hep G2 (Lane 5), Neuro-2a (Lane 6), PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of fixed PC-12 cells stained with 20 µg/mL of Mouse IgM Isotype Control Purified (left) or 20 µg/mL of Anti-Polysialylated Neural Cell Adhesion Molecule (PSA-NCAM) Purified (right), followed by Anti-mouse IgM Biotin and Streptavidin FITC.Nuclei are stained with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of PSA-NCAM was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSA-NCAM Monoclonal Antibody (12E3), eBioscience (Product # 14-9118-82) at 20 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane localization. Panel e represents HepG2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Effect of DPCs on neurogenesis in adult mouse hippocampal slices in vitro. ( A ) Experimental setup. Mouse hippocampus slices were cultivated on matrigel with DPCs or by themselves for 11 days, followed by immunofluorescence staining; ( B ) Images were taken by a phase contrast microscope. Hippocampus slice-derived cells showed glia cell-like morphology when cultivated alone (left). However, when the hippocampus slice co-cultivated with DPCs, the derived cells exhibited neuron-like morphology and formed networks. Scale bar = 50 um ( C ) Immunofluorescence staining showed that cells derived from the co-cultivated hippocampus slice are positive to the mature neuron mark NeuN (right). However, cells derived from the single cultivated hippocampus did not react with NeuN (left). Images were taken by LSM. Scale bar = 25 um; ( D ) In solo or co-cultivated hippocampus slices, cells reacted with NeuN and PSA-NCAM (a marker of developing and migrating neurons) antibodies. Images were taken by LSM. Scale bar = 50 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Representative x40 bright-field micrographs of the hippocampal dentate gyri of 3 x Tg mice with x100 insets ( A , B ). The subgranular zone (SGZ) located polysialylated neuronal cell adhesion molecule (PSA-NCAM) positive cells are marginally present in vehicle-treated animals ( A ) but show much greater incidence in norvaline-treated mice with penetration into the granule cell layer ( B ). The treatment is associated with a significant increase in the PSA-NCAM immunopositive area ( C ) and stain intensity ( D ). Scale bars 50 um, insets 10 um. The data are presented as means +- SEM. * p < 0.05, ** p < 0.01, (two-tailed Student's t -test), n = 8.

14-9118-82

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