Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 56-0566-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD56 (NCAM) Monoclonal Antibody (TULY56), Alexa Fluor 700, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis. Applications Tested: This TULY56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Antibody clone number
- TULY56
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Memory B Cells Activate Brain-Homing, Autoreactive CD4+ T Cells in Multiple Sclerosis.
Jelcic I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, Madjovski A, Ruhrmann S, Faigle W, Frauenknecht K, Pinilla C, Santos R, Hammer C, Ortiz Y, Opitz L, Grönlund H, Rogler G, Boyman O, Reynolds R, Lutterotti A, Khademi M, Olsson T, Piehl F, Sospedra M, Martin R
Cell 2018 Sep 20;175(1):85-100.e23
Cell 2018 Sep 20;175(1):85-100.e23
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0037-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-80) (left) or CD56 Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Near infrared dye