60021AD

antibody from STEMCELL Technologies Inc

Targeting: NCAM1 CD56, NCAM

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60021AD

Provider

STEMCELL Technologies Inc

Product name

Anti-Human CD56 Antibody, Clone HCD56, Alexa Fluor® 488

Antibody type

Monoclonal

Description

The HCD56 antibody reacts with CD56 (NCAM), a member of the immunoglobulin (Ig) super family containing 5 Ig-like domains and two fibronectin type-3 domains in its extracellular portion. Multiple isoforms have been identified, including transmembrane and GPI-anchored forms. CD56 is subject to several types of post-translational modification, including addition of a polysialic acid moiety to the 5th Ig-like domain. The ~140 kDa isoform of CD56 is considered the prototypic cell surface marker on human NK cells. Around 90% of the NK cells express CD56 at low density while ~10% express the protein at relatively high levels. CD56 is also variably expressed by a subset of NK-T cells in peripheral blood, neurons, muscle cells, some stem cells, and in vitro-cultured T cell clones. It is also expressed by several types of tumors, including myeloma, neuroblastoma and small cell lung carcinoma cells. CD56 mediates cell-cell and cell-matrix adhesion via homophilic and heterophilic interactions, respectively, and has been implicated in activating intracellular signaling pathways. CD56 plays important roles in neuronal development both during embryogenesis and in adults. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA) and EasySep™ Human CD56 Positive Selection Kit (Catalog #18055); partial blocking may be observed, as well as EasySep™ HLA CD2 Positive Selection Kit (Catalog #18657HLA) and EasySep™ Human NK Cell Enrichment Kit (Catalog #19055).

Reactivity

Human

Host

Human

Isotype

IgG

Vial size

100 Tests

Storage

2 - 8°C