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- Product number
- A00184-4 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-NCAM1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for NCAM1 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Butylparaben disordered intestinal homeostasis in Chinese striped-necked turtles (Mauremys sinensis).
Bell's palsy was associated with TRPV2 downregulation of Schwann cell by cold stress.
In vitro hematopoietic differentiation of human embryonic stem cells induced by co-culture with human bone marrow stromal cells and low dose cytokines.
Ding L, Wu X, Lin J, Zhang J, Shi H, Hong M, Fang Z
Ecotoxicology and environmental safety 2023 Jun 29;262:115193
Ecotoxicology and environmental safety 2023 Jun 29;262:115193
Bell's palsy was associated with TRPV2 downregulation of Schwann cell by cold stress.
Bai Y, Hao W, Zeng Z, Zhang T, Zhang W, Yang J, Wu F, Li X
Journal of stomatology, oral and maxillofacial surgery 2023 Dec;124(6S):101533
Journal of stomatology, oral and maxillofacial surgery 2023 Dec;124(6S):101533
In vitro hematopoietic differentiation of human embryonic stem cells induced by co-culture with human bone marrow stromal cells and low dose cytokines.
Wang J, Zhao HP, Lin G, Xie CQ, Nie DS, Wang QR, Lu GX
Cell biology international 2005 Aug;29(8):654-61
Cell biology international 2005 Aug;29(8):654-61
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of NCAM1 using anti-NCAM1 antibody (A00184-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 antigen affinity purified polyclonal antibody (Catalog # A00184-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCAM1 at approximately 150KD. The expected band size for NCAM1 is at 150KD.
- Additional image
