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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ6330 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NCAM1 Antibody / CD56
- Antibody type
- Polyclonal
- Description
- This highly specific NCAM1 antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NCAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse Neuro-2a lysate with NCAM1 antibody. Predicted molecular weight: ~110 kDa (soluble fragment), ~120/125 kDa (GPI-anchored), 140/180 kDa (transmembrane isoforms).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with NCAM1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human 293T cells with NCAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCAM1 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-2 OS cells with NCAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCAM1 antibody.
